anti par  (Cell Signaling Technology Inc)

Journal: Frontiers in Nutrition

Article Title: Shikimic acid attenuates oxidative stress-induced senescence in 3D4/21 cells by modulating PARP1 activity and DNA damage response

doi: 10.3389/fnut.2025.1614148

Figure Lengend Snippet: Effect of shikimic acid on XRCC1, PARP1 protein expression and PAR levels ( n = 3, where 3 is the number of biological replicates per group). (A) Representative Western blot images for XRCC1, PARP1, and PAR levels. (B) Effect of SA on XRCC1 protein expression in 3D4/21 cells. (C) Effect of SA on PARP1 protein expression in 3D4/21 cells. (D) Effect of SA on 3D4/21 cells PAR levels.

Article Snippet: Membranes were then incubated with primary antibodies against XRCC1 (Abcam, ab9147, Cambridge, UK), PARP1 (Affinity Biosciences, DF7198, Cincinnati, USA), and PAR (Cell Signaling Technology, CST 83732, Massachusetts, USA) at 4 °C for 12 h. Subsequently, membranes were incubated with appropriate secondary antibodies for 2 h at room temperature.

Techniques: Expressing, Western Blot

Journal: The EMBO Journal

Article Title: PARP7 is a proteotoxic stress sensor that labels proteins for degradation

doi: 10.1038/s44318-025-00545-7

Figure Lengend Snippet: ( A ) HeLa cells were treated with 10 µM deubiquitinase inhibitor (DUBi) PR-619 for 30 min, followed by fixation in methanol and staining using a PAR/MAR antibody from Cell Signaling Technology (E6F6A). The mounted cells were imaged using a confocal microscope. ( B ) As in ( A ), with fixation of the cells in glyoxal. ( C , D ) As in ( A , B ), using a mono(ADP-ribose)-specific antibody from BioRad (HCA354). Scale bars represent 20 µm. To enhance the visibility of foci, grayscale images of the MAR/PAR or MAR signals were inverted and shown on the left of each panel. On the right of each panel, merged images displaying DNA in blue and MAR/PAR or MAR signal in green.

Article Snippet: Antibodies used: rabbit anti-ADPr (HCA354, BioRad), rabbit anti-MAR/PAR E6F6A (CST, lot 5; Cat 83732S), mouse anti-ubiquitin P4D1 (Santa Cruz, sc-8017, Lot D0123), mouse anti-mono and polyubiquitinated conjugates FK2 (BIOZOL, Cat UBI-68-0122-100), mouse anti-Tubulin-alpha (Santa Cruz, sc-5286, Lot L1522), mouse anti-p62 (Santa Cruz, sc-28359, Lot H2621), rabbit anti-GFP (Chromotek, Cat pabg1, Lot 70828032AB), mouse anti-HA (Covance, Cat MMS-101R), mouse anti-LC3B (G-9) (Santa Cruz, Cat sc-376404, Lot G2523), Rabbit anti-LC3B (CST, Cat 2775S, Lot 14), mouse anti-GAPDH (Santa Cruz, Cat sc-32233, Lot J2523) and rabbit anti-c-FOS (66590-1-Ig, Proteintech).

Techniques: Staining, Microscopy